Rotated canonical correlation analysis for multilingual corpora

This paper aims at proposing the joint use of Canonical Correlation Analysis and Procrustes Rotations (RCA), when we deal with a text and its translation into another language. The basic idea is representing words in the two different natural languages on a common reference space. The main characteristic of this space is to be lan-guage independent, although Procrustes Rotation is performed transforming the lexical table derived from trans-lation by minimizing its distance from the lexical table belonging to the original corpus, while the subsequent Canonical Correlation Analysis treats symmetrically the two word sets. The most interesting RCA feature is building a unique reference space for representing the correlation structure in the data, inducing the two systems of canonical factors to lie on the same space. These graphical representations enables us to read distances be-tween corresponding points in terms of different way of translating the same word in relation with the general context defined by the canonical variates. Trying to understand the distances between matched points could rep-resent an useful tool for enriching lexical resources in a translation procedure. In this paper we propose the com-parison of the most frequent content bearing words in the two languages, analyzing one year (2003) of Le Monde Diplomatique and its Italian edition

A cosine based validation measure for Document Clustering

Document Clustering is the peculiar application of cluster analysis methods on huge documentary databases. Document Clustering aims at organizing a large quantity of unlabelled documents into a smaller number of meaningful and coherent clusters, similar in content. One of the main unsolved problems in clustering literature is the lack of a reliable methodology to evaluate results, although a wide variety of validation measures has been proposed. If those measures are often unsatisfactory when dealing with numerical databases, they definitely underperform in Document Clustering. This paper proposes a new validation measure. After introducing the most common approaches to Document Clustering, our attention is focused on Spherical K-means, do to its strict connection with the Vector Space Model, typical of Information Retrieval. Since Spherical K-means adopts a cosine-based similarity measure, we propose a validation measure based on the same criterion. The new measure effectiveness is shown in the frame of a comparative study, by involving 13 different corpora (usually used in literature for comparing different proposals) and 15 validation measures

A polarity-based strategy for ranking social media reviews

TheOpinionMining methods are widely used to analyse and classify the choices, preferences and behaviours of consumers through the opinions gathered on the Web. On social media like TripAdvisor such opinions are usually expressed with a score and a short text. This paper proposes a strategy for ranking reviews using a scale based jointly on the rating and on the text of the reviews

Heat shock proteins levels and expression in chronic obstructive pulmonary disease and vernal keratoconjunctivitis

Inflammatory response in different organs share many similarities, but site-specific signs. Symptoms can be related to mucosal structure changes. The aim of the study was to compare heat shock proteins (HSPs) levels and expression in chronic obstructive pulmonary disease (COPD) to other inflammatory status of mucosa, such as vernal keratoconjunctivitis (VKC), a recurrent ocular inflammatory disease in which autoimune aggression may have a pathogenetic role. We examined bronchial mucosal biopsies from COPD patients (moderate to severe stage) and conjunctival biopsies from VKC patients; age-matched controls were selected for each group. We evaluated levels (by immunohistochemistry) and expression (by RT-PCR) of a panel of HSPs, among which Hsp10, Hsp27, Hsp40, Hsp60, Hsp70, Hsp90, and of the main heat shock transcription factor (both HSF-1 and pHSF-1). Hsp10 levels and expression increased in all pathological conditions, Hsp27 in VKC, Hsp40 in COPD and VKC, Hsp60 in COPD, Hsp70 and Hsp90 in VKC, as compared to their appropriate controls. Transcription factor pHSF-1 positive cells were significantly increased in COPD compared to controls, while was unaltered in VKC. Moreover, all pathological tissues showed increased levels of macrophages (CD68 positive) in lamina propria, COPD showed increased levels of neutrophils (elastase positive) and VKC increased levels of eosinophils (EG2 positive). Finally, Hsp60 colocalize with elastase positive cells in COPD. These results indicate that HSPs levels and expression change during development of different types of inflammation. Further studies will prove their active involvement and functions in triggering and/or maintaining the inflammatory status

Novel in vitro and in vivo data on the cellular localization of Hsp10 in smokers affected by COPD and in lung-derived cell lines exposed to cigarette smoke extract as stressor

Cigarette smoke is a potent stressor for the respiratory system, contributing to pathogenesis, for instance in chronic obstructive pulmonary disease (COPD), but its effects on the expression, function, and cellular localization of mitochondrial chaperonins are still largely unknown. We studied in vivo (airways biopsies) the localization of Hsp10 and Hsp60 in patients (smokers and non-smokers) affected by mild-moderate COPD, and characterized the effects of non-lethal doses of cigarette smoke extract (CSE) on the expression of these molecules in two human cell lines: lung fibroblasts (HFL-1) and bronchial epithelial (16HBE). We applied various in vitro methods: immunohistochemistry (IHC), subcellular fractionation analyses (SFA), Western blotting (WB), immunocytochemistry (ICC), and transmission electron microscopy (TEM) immunogold, and used bioinformatics and databases searches to gather structural in silico data for interpreting and complementing the in vitro results. IHC showed that in smokers and non-smokers COPD patients Hsp10 was localized in both, the cytoplasm and the nucleus of epithelial and lamina propria cells, while Hsp60 was present only in the cytosol. ICC, SFA, and WB on both CSE-exposed cell lines confirmed the presence of nuclear Hsp10, with an increasing trend in parallel to CSE concentration. TEM immunogold further confirmed Hsp10 in the nucleus, in addition to its presence in the cytoplasm and mitochondria, on both cell lines. Bioinformatics and in silico structural analyses indicated that Hsp10 can localize in extramitochondrial sites, such as the nucleus, even if Hsp10 lacks known DNA-binding motifs or nuclear import signals in its primary sequence. Our data suggest a link between exposure to exogenous oxidative stress and cell response, involving Hsp10, which would play roles different from its canonical functions. It is known that Hsp10 can display an array of functions depending on its location: cytoplasm, mitochondria, or extracellular. Here, we show for the first time the presence of Hsp10 in the nucleus of epithelial and stromal human-lung cell lines, paralleling the observations in vivo in COPD patients, and indicating that intranuclear Hsp10 levels are affected by oxidative stress due to an exogenous stressor like cigarette-smoke. The questions now are by what mechanism Hsp10 becomes a resident of the nucleus and what are its functions there.

Nuclear localization and new isoforms detection give new insights on Hsp10 functions in normal and cigarette smoke-stressed lung cells

Heat-shock protein (Hsp)10 is the co-chaperone for Hsp60 inside mitochondria, but it also resides outside the organelle. Variations in its levels and intracellular dis- tribution have been documented in pathological conditions, e.g. cancer and chronic obstructive pulmonary disease (COPD). Cigarette smoke (CS) is a potent stressor for the respiratory system, but its effects on the expression, function, and cellular locali- zation of mitochondrial chaperonins are still largely unknown. We studied in vivo (airways biopsies) the localization of Hsp10 and Hsp60 in patients (smokers and non-smokers) affected by mild-moderate COPD, and charac- terized the effects of non-lethal doses of CS extract (CSE) on the expression of these molecules in two human cell lines: lung fibroblasts (HFL-1) and bronchial epithelial cells (16HBE). We applied various in vitro methods: IHC, subcellular fractionation analyses (SFA), western blotting (WB), ICC, transmission electron microscopy (TEM) immunogold, chromati protein extracts (CPE), as well as 2D-gel based proteomics analyses. Bioinformatics was used to gather structural in silico data. IHC showed that Hsp10 occurred in nuclei of epithelial and lamina propria cells of bronchial mucosa from non-smokers and smokers. ICC, SFA, and WB showed that 16HBE and HFL-1 cells featured nuclear Hsp10, before and after CSE exposure; TEM immunogold further confirmed this observation. Proteomics data showed that CSE stimulation did not increase the levels of Hsp10 but did elicit qualitative changes as indicated by molecular weight and isoelectric point shifts. Bioinformatics analyses indicated that Hsp10 can localize in extramitochondrial sites, such as the nucleus, even if Hsp10 lacks known DNA-binding motifs or nuclear import signals. Hsp10 nuclear levels increased after CSE stimulation in HFL-1, indicating cytosol to nucleus migration, and although Hsp10 did not bind DNA, it bound a DNA-associated protein as suggested by CPE/gel retardation experiments. Data reported here indicate that in human cells of the respiratory mucosa there are at least three different intracellular locales for Hsp10: mitochondrial, nuclear, and cyto- solic. Further experiments are en route for the definition of the mechanisms underlying the transfer of Hsp10 to the nucleus and other cellular/extracellular compartments. This work was supported by grants from University of Palermo (FFR 2012) to GLR

To be a Statistician and a Social Scientist in Europe: the German, Dutch and Norwegian experiences

The lecture has been devoted to the different opportunities for young statisticians to work in Europe, focusing on the German, the Dutch snd the Norwegian experience

Textual Data Analysis for Open-Questions in Repeated Surveys

The paper addresses the problem of analysing answers to an open question observed in different waves of a repeated survey. Multiway data analysis techniques can offer interesting tools for doing that. Unfortunately, dealing with textual data some peculiar problems arise. The aim of this paper is to propose the use of non-symmetrical data analysis techniques in order to follow lexical behaviours with respect to a set of explanatory numerical variables, defining groups through time. Furthermore, attention is paid to the definition of a conjoint vocabulary